Testosterone, Total, Serum, Mass Spectrometry

CPT: 84403

Expected Turnaround Time

3 - 5 days

Specimen Requirements


Specimen

Serum

Volume

0.8 mL

Minimum Volume

0.4 mL (Note: This volume does not allow for repeat testing.)

Container

Tiger-top (SST) tube or red-top tube

Collection

Separate within one hour; transfer into a plastic transport tube.

Storage Instructions

Ship refrigerated or frozen on dry ice.

Test Details


Methodology

High-pressure liquid chromatography/tandem mass spectrometry (HPLC/MS-MS)

Reference Interval

Premature Infants Male Range(ng/dL) Female Range(ng/dL) 26-28 weeks Day 4 59-125 5-16 31-35 weeks Day 4 37-198 5-22 Newborns Male Range(ng/dL) Female Range(ng/dL) Newborns 75-400 20-64 Male 1-7 Months: Levels decrease rapidly the first week to 20 - 50, then increase to 60 - 400 between 20 - 60 days. Levels then decline to prepubertal range levels of <2.5 - 10 by 7 months. Female 1-7 Months: Levels decrease during the first month to less than 10 and remain there until puberty. Prepubertal Children Males (1-10 Years): <2.5-10 Females (1-9 Years): <2.5-10 Puberty Tanner Stage Age (years) Male 1 <9.8 2.5-10 2 9.8-14.5 18-150 3 10.7-15.4 100-320 4 11.8-16.2 200-620 5 12.8-17.3 350-970 Tanner Stage Age (years) Female 1 <9.2 <2.5-10 2 9.2-13.7 7-28 3 10.0-14.4 15-35 4 10.7-15.6 13-32 5 11.8-18.6 20-38 Adults Range(ng/dL) Males >18 years 264-916 This LabCorp LC/MS-MS method is currently certified by the CDC Hormone Standardization Program (HoST). Adult male reference interval is based on a population of healthy nonobese males (BMI <30) between 19 and 39 years old.1 Premenopausal females 10-55 Postmenopausal females 7-40

References

1. Travison TG, Vesper HW, Orwoll E, et al. Harmonized reference ranges for circulating testosterone levels in men of four cohort studies in the United States and Europe. J Clin Endocrinal Metab 2017 April; 102(4):1161-1173. doi:10.1210/jc.2016-2935