Synonyms
- 11-Deoxycortisol
- Compound S
Special Instructions
For information on metyrapone testing, consult the Metyrapone Stimulation section of the Endocrine Appendix.
Expected Turnaround Time
6 - 15 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Specimen Requirements
Specimen
Serum (preferred) or plasma
Volume
1 mL
Minimum Volume
0.2 mL
Container
Gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube
Collection
Serum/plasma must be separated from cells within 45 minutes of venipuncture. Send serum/plasma in a plastic transport tube.
Storage Instructions
Freeze.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 1 day |
Refrigerated | 1 day |
Frozen | 2 years |
Freeze/thaw cycles | Stable x3 |
Test Details
Use
Evaluate hypothalamic-pituitary-adrenal axis and pituitary ACTH reserve. 11-Deoxycortisol is the immediate precursor of cortisol and follows the same catabolic pathways as cortisol.
Methodology
Liquid chromatography/tandem mass spectrometry (LC/MS-MS) after chromatography and solvent extraction
Reference Interval
See table.
Premature Infants | |
|---|---|
26 to 28 wk, day 4 | 110−1376 ng/dL |
31 to 35 wk, day 4 | 48−579 ng/dL |
Full-term Infants | |
3 d | 13−147 ng/dL |
31 d to 11 m | <10−156 ng/dL |
Prepubertal Children | |
8 AM | 20−155 ng/dL |
Pubertal Children and Adults | |
8 AM | 12−158 ng/dL |
Additional Information
A primary use of 11-desoxycortisol testing is to detect congenital adrenal hyperplasia due to steroid 11-β-hydroxylase deficiency. This is a genetic disease caused by a deficiency of the CYP11B1 gene. 11-β-hydroxylase deficiency is transmitted as an autosomal recessive trait. It is associated with low-renin hypertension, hypokalemia, excess androgens (especially androstenedione), and genital ambiguity in affected females. The differential biochemical diagnosis of congenital adrenal hyperplasia caused by 11-β-hydroxylase deficiency includes finding increased serum concentrations of deoxycorticosterone, 11-desoxycortisol and androstenedione, and suppressed plasma renin concentrations. The disorder may be treated by administration of glucocorticoids. An additional use is to evaluate hypothalamic-pituitary-adrenal axis and pituitary ACTH reserve. 11-deoxycortisol is the immediate precursor of cortisol and follows the same catabolic pathways as cortisol.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 500171 | 11-Desoxycortisol | 1657-6 | 504584 | 11-Desoxycortisol | ng/dL | 1657-6 |