11-Deoxycortisol

CPT: 82634

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Synonyms

11-Desoxycortisol
Compound S for Metyrapone Test

Expected Turnaround Time

6 - 10 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

ACTH Stimulation Test

Specimen Requirements

Specimen

Serum, frozen

Volume

0.5 mL

Minimum Volume

0.2 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube or gel-barrier tube

Collection

Transfer the serum into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp N° 49482). Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze.

Stability Requirements

Temperature	Period
Room temperature	1 day
Refrigerated	1 day
Frozen	2 years
Freeze/thaw cycles	Stable x3

Patient Preparation

No isotopes administered 24 hours prior to venipuncture

Causes for Rejection

Recently administered isotopes

Test Details

Use

Evaluate hypothalamic-pituitary-adrenal axis and pituitary ACTH reserve

Methodology

Liquid chromatography/tandem mass spectrometry (LC/MS-MS) after chromatography and solvent extraction

Additional Information

11-Deoxycortisol is the immediate precursor of cortisol and follows the same catabolic pathways as cortisol. The conversion of 11-deoxycortisol to cortisol is inhibited by metyrapone, which acts on 11-β-hydroxylase. The metyrapone test (see the online Endocrine Appendix: ACTH Stimulation) serves as a reliable and sensitive indicator of pituitary ACTH secretory reserve. The 11-deoxycortisol levels normally increase to 100 times the control value following metyrapone administration. Reduced response occurs in patients with hypoadrenalism or with hypopituitarism and in some patients with diseases of the hypothalamus. Patients with myxedema, some pregnant patients, and those on oral contraceptives respond poorly.

References

Sindler BH, Griffing GT, Melby JC, et al. The superiority of the metyrapone test versus the high-dose dexamethasone test in the differential diagnosis of Cushing's syndrome. Am J Med. 1983 Apr; 74(4):657-662. 6837591

Spark RF. Simplified assessment of pituitary-adrenal reserve: Measurement of serum 11-deoxycortisol after metyrapone. Ann Intern Med. 1971 Nov; 75(5):717-723.4330677

LOINC® Map

Order Code	Order Code Name	Order Loinc	Result Code	Result Code Name	UofM	Result LOINC
500550	11-Desoxycortisol	1657-6	500551	Desoxycortisol(Compound S),S	ug/dL	1657-6